Project

Part:BBa_K1894001:Design

Designed by: Qinyu Ge   Group: iGEM16_Nanjing_NFLS   (2016-10-13)


shuttle plasmid which can replicate in cyanobacteria and E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5892
    Illegal NotI site found at 6620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 6613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1596
    Illegal NgoMIV site found at 1756
    Illegal NgoMIV site found at 4803
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2676


Design Notes

We introduce shuttle plasmid into our experiments because we need to transform both E.coli and microcystis aeruginosa during experiments. （The plasmid of microcystis aeruginosa itself does not contain selectable marker and cannot replicate in E.coli. Traditional plasmid vector used to transform E.coli cannot transform microcystis either.


Source

Ori site of cyanobacterium plasmid comes from indigenous plasmids pPbs extracted from Plectonema boryanum.

References